Culture media for petri dishes
- 🧫 PRE-FILLED CULTURE MEDIA: Ideal for fungal cultures and cloning, with sterilized agar.
- 💎 ADVANTAGES: Ease of use and reduction of risk of contamination.
- 🔬 DIFFERENT ENVIRONMENTS:
- “MPA” medium (Malt, Peptone, Agar-agar): Optimal multiplication of most mycelia.
- Medium "Sabouraud + Antibiotic": Selective to limit bacteria, ideal for cloning and germination of spores.
- 🌍 SEPARATE AGAR-AGAR: Available to create your own mixtures and enrich your culture media.
- 📏 USE: Dissolve 47 g of medium in 1 liter of hot water, sterilize at 121°C for 20-30 minutes, leave to cool, then distribute into the boxes.
Discover our culture media for preparing your petri dishes:
-
"MEA+" Medium (Malt Extract, Agar-agar, Peptone):
Rich nutrient medium, suitable for culturing most mycelia. It also promotes bacterial growth, making it a good choice for testing air or sample purity (contamination tests). - "Sabouraud + Antibiotic" medium:
Enriched with an antibiotic (chloramphenicol), this medium limits bacterial growth and becomes very selective. It is ideal for sensitive manipulations such as cloning wild fungi or spore germination, especially under non-sterile conditions. However, mycelial growth may be somewhat slower than with a medium like MEA+.
These media are designed to be used with our sterile petri dishes and our stretch films.
Usage:
- Dissolve the 47 g packet in 1 liter of hot water (preferably distilled or demineralized water). Mix thoroughly.
- Sterilize at 121°C (15 PSI) for 20-30 minutes.
- Let cool to 45-50°C, then distribute into the petri dishes.
Cooling and distributing the agar in the petri dishes must be done in a sterile environment (laminar flow hood, Bunsen burner, or other technique) to avoid contamination.
If you pour the liquid too hot into the petri dishes, condensation may form on the lids. If you wait too long to cool, the mixture may solidify.