Culture media for petri dishes

Medium "MPA" (Malt, Peptone, Agar):

This is a classic medium ideal for the propagation of most mycelium.

Medium "Sabouraud + Antibiotic":

By adding an antibiotic (chloramphenicol), bacterial growth is limited, making it very selective. This recipe is generally used for more risky tasks, such as cloning mushrooms from the wild or spore germination. Mycelium growth is slower compared to the MPA medium.

These media are designed to be used with our sterile petri dishes and stretch films.

Usage:

• Dissolve the 47 g sachet in 1 liter of hot water (preferably distilled or demineralized water). Homogenize.
• Sterilize at 121°C (15 PSI) for 20-30 minutes.
• Allow to cool to 45-50°C, then distribute into petri dishes.

Cooling and pouring the agar into the petri dishes must be done in a sterile environment (laminar flow hood, Bunsen burner, or other techniques) to avoid contamination.

If you pour the liquid too hot into the petri dishes, condensation may form on the lids. If you wait too long for cooling, the mixture may solidify.

Agar-Agar:

If you want to create your own mixture, we sell agar-agar separately. According to recipes, it is generally recommended to use between 20 and 25 g per liter.

Enrich your culture media with specific ingredients such as potato extract, malt, dog food pellets, manure extract, sugar, sawdust, etc.

It is also possible to make a "Water Agar" medium without enrichment, which is useful for certain laboratory tasks such as strain selection or purification.